TechTalk – Live “Street View” of Intracellular Organelles’ Interactions
November 13 2023 (Monday) 3:00-4:00pm
The number of colors in fluorescence microscopy is far less than the types of intracellular compartments. I will present our recent progress in super resolution imaging and deep convolutional neuronal networks to segment 15 subcellular structures. This approach bypasses the limitations of multi-color imaging, accelerates the imaging speed by one order of magnitude, and can accurately segment vesicle organelles with similar shapes and sizes. The super-resolution advantages were demonstrated in resolving the 3D anatomic nanostructures at different mitotic phases and tracking the fast dynamic interactions among nine intracellular compartments in live cell. We show transfer learning ability of our networks among different microscopes, different cell types, and even complexed system of living tissues.










