In the past 10+ years, laser microscopy has successfully made it permeated not only in biochemistry and cell/molecular biology research, but also in numerous preclinical and clinical applications. However, our understanding of health and disease is still very limited. This lecture will introduce the latest breakthrough in laser microscopy technologies developed at HKU that can address some of these challenges. Especially these technologies can generate unprecedented views and understanding of the living biological cells. They include: capturing high-resolution motion picture of the swift-flying brain signals in a living animal; visualizing the inner workings of biological cells and organisms in 3D without killing them; and detecting rare cancer cells in millions of blood cells. Not only can these technologies impact new biological discovery (e.g. neuroscience), but also creating many new opportunities in cost-effective clinical diagnosis, especially cancer screening.