“On-the-fly” imaging bioassay platform for cost-effective biomedical diagnosis

Optical microscopy is now seamlessly integrated with many bioassay technologies for disease diagnosis. However, current techniques lack the throughput to image large and heterogeneous cell and tissue samples systematically. In addition, the vast majority of the methods overwhelmingly rely on biochemical markers such as stains and antibodies for enhancing image contrast, which are however not always be cost effective and efficient. To address these challenges, this project is to develop a spinning disk bioassay platform, which enables ultralarge-scale, label-free, high-resolution “on-the-fly” single-cell or whole-tissue-slide imaging at an imaging rate of 100-times faster than current assays. This high-throughput and high-content technology could open a new paradigm in data-driven bioassay applied in disease diagnostics, and biotechnology industries.